The screen took place over two days - each plate was screened in technical duplicate both in co-culture with macrophages (MO) and in monoculture under tissue culture conditions (TC). For the TC screen, only phase images were acquired. For the MO screen, co-cultures were stained using a FITC-conjugated anti-Candida albicans antibody therefore phase and FITC channel images were acquired. Each mutant has 6 corresponding images: 2 phase images from the TC screen (one per replicate) - 2 FITC channel images from the MO screen - and 2 merge (phase + FITC) images from the MO screen. GRACE plates 1-17 were screened in duplicate on one day - alongside one replicate of plate 25 and GRACE plates 18-24 were screened in duplicate on another day - alongside the second replicate of plate 25.